Author: John P. Aris, Wesley E. Bolch, Madison Bushloper, Lauren E Ellis, Adam Grey Haneberg, Elizabeth Martin, Bonnie N. C. President, Andrew Robert Sforza, Alexander Zorrilla 👨🔬
Affiliation: Johns Hopkins University, Biomedical Engineering, University of Florida 🌍
Purpose: To develop six additional high-fidelity 3D models of kidney renal cortical labyrinth from H&E-stained histology slides to support radiation dosimetry in radiopharmaceutical therapies (RPTs).
Methods: A prototype model of renal cortex tissue was created by assembling 31 H&E-stained histology slides consecutively to illustrate progression through the thickness of the renal tissue. Each slide was then imaged using the ProGres camera connected to the Zeiss Axiophot Microscope at 10x. These images were then imported into 3D Slicer to produce a tissue volume where the convoluted tubules and renal corpuscles were manually segmented. The final polygon mesh model was created in Rhinoceros 3D with a built-in module called Grasshopper. Each glomerulus was scaled to achieve the maximum diameters measured on the histology slides with the Bowman’s Capsule scaled outwards to enclose each glomerulus. All proximal convoluted tubules (PCTs) and distal convoluted tubules (DCT) were created using the multi-pipe function in Grasshopper using central points segmented from 3D slicer. This methodology was reproduced to create a separate microscale model of the kidney cortex for six unique regions of interest in the slide set.
Results: Six additional models were successfully created to complete a series of seven microscale models of the renal cortex. Each model varies in the number of renal corpuscles, convoluted tubules, and extracellular/residual space. S-Values were computed for various source-region combinations for monoenergetic electrons and alphas for several radionuclides based on literature descriptions of dose deposition in the renal cortex. This series provides a full statistical analysis.
Conclusion: Six additional histology-based 3D models of the renal cortical labyrinth were successfully developed. S-Values were computed for this model to facilitate dosimetric advancement in RPTs on a microscopic scale.